Cisplatin-Protein Interactions: Unexpected Drug Binding to N-Terminal Amine and Lysine Side Chains.
Identifieur interne : 000120 ( Main/Exploration ); précédent : 000119; suivant : 000121Cisplatin-Protein Interactions: Unexpected Drug Binding to N-Terminal Amine and Lysine Side Chains.
Auteurs : Irene Russo Krauss [Italie] ; Giarita Ferraro [Italie] ; Antonello Merlino [Italie]Source :
- Inorganic chemistry [ 1520-510X ] ; 2016.
Descripteurs français
- KwdFr :
- Cisplatine (composition chimique), Cisplatine (métabolisme), Conformation des protéines (MeSH), Cristallographie aux rayons X (MeSH), Hydrolyse (MeSH), Lysine (métabolisme), Protéines végétales (composition chimique), Protéines végétales (métabolisme), Sites de fixation (MeSH), Spectrophotométrie UV (MeSH).
- MESH :
- composition chimique : Cisplatine, Protéines végétales.
- métabolisme : Cisplatine, Lysine, Protéines végétales.
- Conformation des protéines, Cristallographie aux rayons X, Hydrolyse, Sites de fixation, Spectrophotométrie UV.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Cisplatin, Plant Proteins.
- chemical , metabolism : Cisplatin, Lysine, Plant Proteins.
- Binding Sites, Crystallography, X-Ray, Hydrolysis, Protein Conformation, Spectrophotometry, Ultraviolet.
Abstract
Literature studies carried out by mass spectrometry and X-ray crystallography have demonstrated that cisplatin is able to bind proteins mainly close to Met, His, and free Cys side chains. To identify possible alternative modes of cisplatin binding to proteins at the molecular level, here we have solved the high-resolution X-ray structure of the adduct formed in the reaction between the drug and the model protein thaumatin, which does not contain any His and free Cys residues and possesses just one buried Met. Our data reveal unexpected cisplatin binding sites on the protein surface that could have general significance: cisplatin fragments -[Pt(NH3)2Cl](+), -[Pt(NH3)Cl2], and -[Pt(NH3)2(OH2)](2+) bind to a protein N-terminus and close to Lys and Glu side chains.
DOI: 10.1021/acs.inorgchem.6b01234
PubMed: 27482735
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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I-80126 Naples, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Chemical Sciences, University of Naples Federico II , Via Cintia, I-80126 Naples</wicri:regionArea><wicri:noRegion>I-80126 Naples</wicri:noRegion></affiliation></author><author><name sortKey="Merlino, Antonello" sort="Merlino, Antonello" uniqKey="Merlino A" first="Antonello" last="Merlino">Antonello Merlino</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical Sciences, University of Naples Federico II , Via Cintia, I-80126 Naples, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Chemical Sciences, University of Naples Federico II , Via Cintia, I-80126 Naples</wicri:regionArea><wicri:noRegion>I-80126 Naples</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27482735</idno><idno 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Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Chemical Sciences, University of Naples Federico II , Via Cintia, I-80126 Naples</wicri:regionArea><wicri:noRegion>I-80126 Naples</wicri:noRegion></affiliation></author></analytic><series><title level="j">Inorganic chemistry</title><idno type="eISSN">1520-510X</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Binding Sites (MeSH)</term><term>Cisplatin (chemistry)</term><term>Cisplatin (metabolism)</term><term>Crystallography, X-Ray (MeSH)</term><term>Hydrolysis (MeSH)</term><term>Lysine (metabolism)</term><term>Plant Proteins (chemistry)</term><term>Plant Proteins (metabolism)</term><term>Protein Conformation (MeSH)</term><term>Spectrophotometry, Ultraviolet (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cisplatine (composition chimique)</term><term>Cisplatine (métabolisme)</term><term>Conformation des protéines (MeSH)</term><term>Cristallographie aux rayons X (MeSH)</term><term>Hydrolyse (MeSH)</term><term>Lysine (métabolisme)</term><term>Protéines végétales (composition chimique)</term><term>Protéines végétales (métabolisme)</term><term>Sites de fixation (MeSH)</term><term>Spectrophotométrie UV (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Cisplatin</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cisplatin</term><term>Lysine</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Cisplatine</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cisplatine</term><term>Lysine</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Binding Sites</term><term>Crystallography, X-Ray</term><term>Hydrolysis</term><term>Protein Conformation</term><term>Spectrophotometry, Ultraviolet</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Conformation des protéines</term><term>Cristallographie aux rayons X</term><term>Hydrolyse</term><term>Sites de fixation</term><term>Spectrophotométrie UV</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Literature studies carried out by mass spectrometry and X-ray crystallography have demonstrated that cisplatin is able to bind proteins mainly close to Met, His, and free Cys side chains. To identify possible alternative modes of cisplatin binding to proteins at the molecular level, here we have solved the high-resolution X-ray structure of the adduct formed in the reaction between the drug and the model protein thaumatin, which does not contain any His and free Cys residues and possesses just one buried Met. Our data reveal unexpected cisplatin binding sites on the protein surface that could have general significance: cisplatin fragments -[Pt(NH3)2Cl](+), -[Pt(NH3)Cl2], and -[Pt(NH3)2(OH2)](2+) bind to a protein N-terminus and close to Lys and Glu side chains. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27482735</PMID><DateCompleted><Year>2018</Year><Month>02</Month><Day>20</Day></DateCompleted><DateRevised><Year>2018</Year><Month>02</Month><Day>20</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1520-510X</ISSN><JournalIssue CitedMedium="Internet"><Volume>55</Volume><Issue>16</Issue><PubDate><Year>2016</Year><Month>Aug</Month><Day>15</Day></PubDate></JournalIssue><Title>Inorganic chemistry</Title><ISOAbbreviation>Inorg Chem</ISOAbbreviation></Journal><ArticleTitle>Cisplatin-Protein Interactions: Unexpected Drug Binding to N-Terminal Amine and Lysine Side Chains.</ArticleTitle><Pagination><MedlinePgn>7814-6</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/acs.inorgchem.6b01234</ELocationID><Abstract><AbstractText>Literature studies carried out by mass spectrometry and X-ray crystallography have demonstrated that cisplatin is able to bind proteins mainly close to Met, His, and free Cys side chains. To identify possible alternative modes of cisplatin binding to proteins at the molecular level, here we have solved the high-resolution X-ray structure of the adduct formed in the reaction between the drug and the model protein thaumatin, which does not contain any His and free Cys residues and possesses just one buried Met. Our data reveal unexpected cisplatin binding sites on the protein surface that could have general significance: cisplatin fragments -[Pt(NH3)2Cl](+), -[Pt(NH3)Cl2], and -[Pt(NH3)2(OH2)](2+) bind to a protein N-terminus and close to Lys and Glu side chains. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Russo Krauss</LastName><ForeName>Irene</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Department of Chemical Sciences, University of Naples Federico II , Via Cintia, I-80126 Naples, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ferraro</LastName><ForeName>Giarita</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Department of Chemical Sciences, University of Naples Federico II , Via Cintia, I-80126 Naples, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Merlino</LastName><ForeName>Antonello</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Chemical Sciences, University of Naples Federico II , Via Cintia, I-80126 Naples, Italy.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>08</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Inorg Chem</MedlineTA><NlmUniqueID>0366543</NlmUniqueID><ISSNLinking>0020-1669</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>53850-34-3</RegistryNumber><NameOfSubstance UI="C003427">thaumatin protein, plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>K3Z4F929H6</RegistryNumber><NameOfSubstance UI="D008239">Lysine</NameOfSubstance></Chemical><Chemical><RegistryNumber>Q20Q21Q62J</RegistryNumber><NameOfSubstance UI="D002945">Cisplatin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001665" MajorTopicYN="N">Binding Sites</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002945" MajorTopicYN="N">Cisplatin</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018360" MajorTopicYN="N">Crystallography, X-Ray</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006868" MajorTopicYN="N">Hydrolysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008239" MajorTopicYN="N">Lysine</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011487" MajorTopicYN="N">Protein Conformation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013056" MajorTopicYN="N">Spectrophotometry, Ultraviolet</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>8</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>8</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>2</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27482735</ArticleId><ArticleId IdType="doi">10.1021/acs.inorgchem.6b01234</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Italie</li></country></list><tree><country name="Italie"><noRegion><name sortKey="Russo Krauss, Irene" sort="Russo Krauss, Irene" uniqKey="Russo Krauss I" first="Irene" last="Russo Krauss">Irene Russo Krauss</name></noRegion><name sortKey="Ferraro, Giarita" sort="Ferraro, Giarita" uniqKey="Ferraro G" first="Giarita" last="Ferraro">Giarita Ferraro</name><name sortKey="Merlino, Antonello" sort="Merlino, Antonello" uniqKey="Merlino A" first="Antonello" last="Merlino">Antonello Merlino</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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